A molecularly imprintedpolymer (MIP)-based impedimetric biosensor was developed for theelectrochemical analysis of low-weight biological molecules. Syntheticpolymeric matrices with specific and selective recognition sites, which arecomplementary to the shapes and sizes of the functional groups of analytes, canbe prepared using the molecular imprinting method. In this study, a smallmolecule, tris(hydroxymethyl)aminomethane (TRIS), was used to coat a graphitepencil tip with a TRIS-containing polyacrylamide gel to fabricate a workingelectrode. The electrode modification and performance were evaluated usingcyclic voltammetry and electrochemical impedance spectroscopy. Theelectrochemical properties of the modified electrodes were observed using anelectrochemical cell comprising a Ag/AgCl reference electrode, a Pt wire as thecounter electrode, and a pencil graphite tip as the working electrode using aredox-phosphate buffer solution with different concentrations of TRIS andEthylenediaminetetraacetic acid (EDTA). The I–V and impedance performance of the chemicallymodified graphite pencil-tip electrodes exhibited decreased conductance andincreased impedance correlating with theincrease in TRIS concentration. Thus,MIP-based small-molecule biosensor prototypes can be promising economicalreplacements over other expensive sensors.