Carbon fiber electrodes (CFEs) are commonly used in detection of neurotransmitters like dopamine. Besides, modification of these electrodes with enzymes enables development of biosensors capable of local analysis. Here, CFEs were fabricated using glass capillary tubes. Basically carbon fibers were inserted into glass capillary tubes and then the tubes were pulled using a micro-puller to insulate carbon fibers. Subsequently, the electrode surface was modified with Pt nanoparticles to evaluate the potential of these electrodes in detection of O2 and H2O2. In the future, these electrodes will be used for construction of biosensors for detection of local ATP.